 |
Viputheshwar Sitaraman
Founder, Blogger
Draw Science.
|
*So what does it mean to 'design' the recognition domain? Essentially, engineered nucleases use one of two kinds of proteins: either zinc fingers (ZFs) or TAL effectors (TALEs). Both recognize sequences of DNA. By putting several different ZFs or several different TALEs together -- and choosing the right ones -- scientists can recognize very specific sequences (ie. the gene for sickle cell anemia) and then cut it out.
The first official "Draw Science" post (other than my rant about science communication)! For more information, see the full text of the article I drew by clicking the link below. Be sure to follow and share science with all your nerdy friends and request any articles you'd like drawn out.
Article: Gaj, T., Gersbach, C., & Barbas, C. (2013). ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering Trends in Biotechnology, 31 (7), 397-405 DOI: 10.1016/j.tibtech.2013.04.004 [Full Text (PDF)]